After reaching the stable phase in culture, A7r5 cells exhibit enhanced activity of creatine kinase (CK) and creatine phosphokinase (CPK). Upon cessation of cell division, A7r5 cells synthesize bone-type creatine kinase and demonstrate creatine phosphokinase (CPK) activity characteristics.

Cell Name: Rat aortic smooth muscle cell line (STR-verified)

Cell Synonyms: A7r5; A7R5

Catalogue No.: TCR-C601

Species: Rat

Tissue Origin: Smooth muscle of thoracic aorta

Disease Characteristics: —

Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS + 1% GlutaMAX + 1% NEAA + 1% P/S

Matching Medium Catalog Number: TCR-G601

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days

Doubling Time: /

Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma contamination