The PK-15 cell line was established in 1955 (Stice, E.). It is a clonal derivative of the PK-1a cell line. This cell line can be used for the propagation and characterization of various viruses. Additionally, electron microscopy has revealed the presence of C-type viral particles within PK-15 cells, making it a useful material for studying C-type viruses.

Cell Name: Porcine kidney epithelial cell line

Cell Synonyms: PK15; PK(15); PK (15); PK 15; PK15; Porcine Kidney-15

Catalogue No.: TCO-C039
Species: Pig
Tissue Origin: Kidney

Disease Characteristics: 一
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G039

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection