The ST cell line was established by Mcclurkin·A·W et al. in 1960. ST cells are commonly used for virus propagation and isolation, and serve as an ideal host for porcine parvovirus (PPV). This cell line can be used for the propagation, assay, and isolation of PPV, TGE (transmissible gastroenteritis), and porcine enteroviruses.

Cell Name: Swine testicular epithelial cell line

Cell Synonyms: ST; Swine Testis; STOMA24; Stoma 24; ST-IOWA; ATCC-ST

Catalogue No.: TCO-C038
Species: Pig
Tissue Origin: Testis
Disease Characteristics: /
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G038

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 72-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection