UMNSAH/DF-1 is a spontaneously immortalized chicken cell line derived from 10-day-old ELL-0 eggs. Primary chicken embryo fibroblasts were isolated and cultured in medium, passaged until senescence. During senescence, centrifugation was performed to maintain cell cultures at 30%–60% confluency. Non-senescent clones were identified and passaged at least 30 times. No clonal proliferation was observed in soft agar, confirming that these cells are immortalized but not transformed. This cell line can be used as a substrate for virus propagation, recombinant protein expression, and recombinant virus production.

Cell Name: Chicken embryo fibroblast cell line

Cell Synonyms: UMNSAH/DF-1; UMNSAH-DF-1; UMNSAH-DF 1; UMNSAH-DF1; UMNSAH/DF#1; DF-1; DF1; Douglas Foster-1

Catalogue No.: TCO-C034
Species: Chicken
Tissue Origin: Embryo
Disease Characteristics: —
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G034
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 22-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection