The CRFK cells are free from Bovine Viral Diarrhea (BVD) virus.
Cell Name: Crandell-Rees feline kidney cell line
Cell Synonyms: CRFK; CrFK; Crfk; Crandell Reese Feline Kidney; Crandell Feline Kidney; CCC; Crandell's Cat Cell
Catalogue No.: TCO-C027
Species: Cat
Tissue Origin: Kidney
Disease Characteristics: Breast cancerMorphology: Epithelial-likeGrowth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS+ 1% P/SMatching Medium Catalog Number: TCO-G027
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 daysDoubling Time: 24-48 hCulture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201Quality Control: Negative for bacteria, fungi, and mycoplasma detection