In September 1958, S.H. Madin and N.B. Darby isolated the MDCK cell line from the kidney of an apparently healthy adult female Cocker Spaniel. Positive for keratin immunoperoxidase staining. MDCK has been used to study the processing of β-amyloid precursor protein and its proteolytic products.

Cell Name: Canine kidney cells

Cell Synonyms: MDCK; MDCK (NBL-2); NBL-2; Madin-Darby Canine Kidney; Madin Darby Canine Kidney

Catalogue No.: TCO-C025
Species: Canine
Tissue Origin: Canine Kidney
Disease Characteristics: —

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G025

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection