This cell line originates from the lung tissue of a female Chinese hamster. Originally named the V cell line, it was renamed V79 by Elkind in 1958 and has been used to study X-ray-induced damage and repair in cultured mammalian cells. This cell line is negative for immunoglobulin production and Epstein-Barr virus expression. It expresses the Fas antigen and is sensitive to TNF and anti-Fas antibodies.

Cell Name: Chinese hamster lung fibroblast cell line

Cell Synonyms: V79; V-79; V 79; Strain V; V79-1; GM00215; GM-215; GM00215A; GM16136; UCW 100

Catalogue No.: TCO-C010
Species: Hamster
Tissue Origin: Lung
Disease Characteristics: —
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G010

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection