Lec1 cells are a mutant strain selected from the proline-deficient CHO cell clone Pro-5 that exhibits resistance to phytohemagglutinin. Lec1 lacks a glycosyltransferase known as GlcNAc-T1, which results in the blockage of N-linked carbohydrates at the Man5GlcNAc2Asn intermediate stage. Lec1 cells are particularly useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or viral glycoproteins, as well as those produced by transfection with cloned DNA.

Cell Name: Hamster ovarian cell line

Cell Synonyms: LEC-1; CHO-Lec1; CHO Lec1; Pro-Lec1.3C; Pro-5 Lec1.3c; Pro-5WgaRI3C

Catalogue No.: TCO-C007
Species: Hamster
Tissue Origin: Ovary

Disease Characteristics: —

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: MEMα + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G007

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection