In 1957, Puck TT established CHO cells from the biopsy tissue of adult Chinese hamster ovaries. CHO-K1 is a subclone of CHO. The growth of CHO-K1 requires proline.

Cell Name: Chinese hamster ovary cell line

Cell Synonyms: CHO-K1; CHO K1; CHOK1; CHO cell clone K1; GM15452

Catalogue No.: TCO-C005
Species: Hamster
Tissue Origin: Ovary

Disease Characteristics: 一
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: F12K + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G005

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection