The DC2.4 cell line is an immortalized mouse bone marrow-derived dendritic cell line. It was generated by transducing bone marrow isolates from C57BL/6 mice with a retroviral vector expressing murine granulocyte-macrophage colony-stimulating factor (GM-CSF) along with the myc and raf oncogenes. DC2.4 exhibits characteristics of dendritic cells, including typical cell morphology, expression of dendritic cell-specific markers, as well as the ability to phagocytose and present exogenous antigens on MHC class I and II molecules. The DC2.4 cell line is widely used in immunological research, particularly in studies related to dendritic cell (DC) biology, immune responses, and T cell activation.

Cell Name: Mouse Dendritic Cells

Cell Synonyms: DC2.4

Catalogue No.: TCM-C832
Species: Mouse

Tissue Origin: Bone Marrow

Disease Feature: Dendritic Cells
Morphology: Round; spindle-shaped, with branched dendrites

Growth Properties: Adherent cells, with a small number of suspension cells

Culture Medium: RPMI-1640 + 10% FBS (fetal bovine serum) + 1% P/S (penicillin/streptomycin)

Passage Ratio: 1:3–1:6; change medium every 2–3 days

Doubling Time: 18–24 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C

Freezing Conditions: 60% complete culture medium + 30% FBS + 10% DMSO; store in liquid nitrogen
Recommended Cryopreservation Solution: HyCyte® One-Step Freezing Medium (ready-to-use, serum-free, no programmed cooling required), Product Code: GUCP-R201

Quality Control: Negative for bacteria, fungi, and mycoplasma detection