The mouse cochlear hair cell line House Ear Institute-Organ of Corti 1 (HEI-OC1) is derived from the cochlea of mice. This cell line is used in research on auditory function in mice and is considered an in vitro system for screening ototoxic drugs. It has been employed to study drug-activated apoptotic pathways, autophagy, senescence, inflammatory responses, cell differentiation, the genetic and epigenetic effects of drugs, as well as the expression of molecular channels and receptors in the cochlea.

Cell Name: Mouse auditory hair cell line

Cell Synonyms: HEI-OC1; HEIOC1; House Ear Institute-Organ of Corti 1

Catalogue No.: TCM-C829
Species: Mouse
Tissue Origin: Mouse Cochlea
Disease Characteristics: /
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCM-G829
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-36 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection