Cell Name: Mouse Sertoli cell line

Cell Synonyms: TM4; TM-4

Catalogue No.: TCM-C828

Species: Mouse

Tissue Origin: Testis

Disease Characteristics: —

Morphology: Epithelial-like

Growth Properties: Adherent

Culture Medium: DMEM/F12 + 2.5% FBS + 5% Horse Serum + 1% P/S

Matching Medium Catalog Number: TCM-G828

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 18 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection