The STC-1 cell line is derived from intestinal tumor tissue of double transgenic mice. These mice were generated by introducing a fusion gene consisting of the rat insulin gene promoter linked to the polyoma virus small T antigen, along with another gene construct combining the rat insulin gene with SV40. These double transgenic mice typically develop intestinal tumors as well as pancreatic β-cell tumors. STC-1 cells are capable of producing the hormone secretin.

Cell Name: Mouse enteroendocrine cell line

Cell Synonyms: STC-1
Catalogue No.: TCM-C827
Species: Mouse
Tissue Origin: Small Intestine
Disease Characteristics: Intestinal
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS (Fetal Bovine Serum) + 1% P/S
Matching Medium Catalog Number: TCM-G827
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: —
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO; store in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (Ready-to-use, serum-free, no programmed cooling required): GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma