The U14 tumor strain was intraperitoneally inoculated into 615 mice, and primary culture was performed using the ascites fluid to establish a cell line.

Cell Name: Mouse cervical carcinoma cell line

Cell Synonyms: U14
Catalogue No.: TCM-C826
Species: Mouse
Tissue Origin: Uterus
Disease Characteristics: Cervical Cancer
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCM-G826
Passage Ratio: 1:2-1:4, 2-3 times per week

Doubling Time: 24-36h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection