This immortalized cell line is derived from the cerebellar tissue of 8-day-old mice and was established by B. Pessac and D. Trisler. The cells exhibit characteristics of microglia. They are GFAP-positive, but no other molecular markers of glial or neuronal cells, including microglia, have been detected.

Cell Name: Mouse cerebellar astrocyte cell line

Cell Synonyms: C8-D1A; C8D1A; Astrocyte type I clone
Catalogue No.: TCM-C825
Species: Mouse
Tissue Origin: Cerebellum
Disease Characteristics: —
Morphology: Neuronal-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G825

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection