Alpha TC1 clone6 is an α-cell line isolated from the pancreas of adenomatous mice in 1988. It was derived by cloning the alpha TC1 cell line, which originated from adenomas in transgenic mice expressing the SV40 large T-antigen oncogene under the control of the rat preproglucagon promoter. This cell line can be used to study various aspects of pancreatic islet biology, including glucagon biosynthesis and cytokine sensitivity.

Cell Name: Mouse pancreatic alpha cell line

Cell Synonyms: Alpha TC1 clone6
Catalogue No.: TCM-C822
Species: Mouse
Tissue Origin: Pancreas
Disease Feature: Adenoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-L+10%FBS+0.1mM NEAA+ 1% P/S
Matching Medium Catalog Number: TCM-G822
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: —Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection