The cell line contains multiple subclones and serves as an excellent in vitro model for studying osteoblast differentiation, particularly the role of ECM signaling pathways.

Cell Name: Mouse preosteoblast cell line

Cell Synonyms: MC3T3-E1; Mc3T3-E1; MC3T3E1; MC-3T3-E1; MC 3T3-E1

Catalogue No.: TCM-C817

Species: Mouse

Tissue Origin: Calvarial bone

Disease Characteristics: —

Morphology: Fibroblast-like

Growth Properties: Adherent

Culture System: MEMα + 10% FBS + 1% Glutamax + 1% Sodium Pyruvate + 1% P/S

Matching Culture Medium No.: TCM-G817

Passage Ratio: 1:4-1:6, with medium renewal every 2-3 days
Doubling Time: 24-32 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection