The mouse immortalized bone marrow-derived macrophage cell line is a cell line obtained through monoclonal screening.

iBMDM is commonly used in macrophage-related research, such as the construction of recombinant adenoviruses for the mouse FcγRⅡB gene and studies on its effects on IL-10 secretion by macrophages.

Cell Name: Immortalized bone marrow-derived macrophage

Cell Synonyms: iBMDM
Catalogue No.: TCM-C809
Species: Mouse
Tissue Origin: Bone Marrow
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCM-G809
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-32 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (Ready-to-use, serum-free, no programmed cooling required), Catalog Number: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma.