$ 1000.00
Mouse myeloblastic leukemia cell line(NFS-60)
Cat.No. Size
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  • Cell Info
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Cat.No.
TCM-C808
Size
1x10^6
Price
$1000.00

Cell Name: Mouse myeloblastic leukemia cell line

Cell Synonyms: M-NFS-60; NFS-60; NFS 60; NFS60

Catalogue No.: TCM-C808

Species: Mouse

Tissue Origin: Peripheral blood

Disease Characteristics: Myeloid leukemia

Morphology: Lymphoblast-like

Growth Characteristics: Suspension

Culture Medium: RPMI-1640 ＋10 ng/mL M-CSF＋0.05 mM β-mercaptoethanol+10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G808

Passage Ratio: 2×105-5×105 cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


Precautions:

  1. When the cell status is good, there is no dependence on M-CSF concentration, and the cells can proliferate normally even in the absence of M-CSF.

  2. When the cells have just been thawed or are in poor condition, the M-CSF concentration can be increased to 10-20 ng/mL to adjust cell activity and promote rapid recovery.


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