Product Name：Luciferase Reporter A20 Cell Line

HyCyte cell line：HyCyte A20-Luc

Catalog No.：TCM-C804L

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：A20

Species：Mouse

Morphology：Lymphoblastoid

Growth properties：Suspension

Culture Medium: RPMI-1640 supplemented with 10 % FBS,4.0 ug/mL Puromycin,0.05 mM 2-mercaptoethanol, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G804L).

Passage：3×10^5-5×10^5cells/mL

Doubling time： 24-48 h

Subculture： Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media： RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).