Cell Name: Mouse B cell lymphoma cell line

Cell Synonyms: A20; A-20

Catalogue No.: TCM-C804

Species: Mouse

Tissue Origin: B lymphocytes

Disease Characteristics: B-cell lymphoma

Morphology: Lymphoblast-like

Growth Characteristics: Suspension

Culture Medium: RPMI-1640 + 10% FBS+0.05 mM 2-mercaptoethanol+ 1% P/S
Matching Medium Catalog Number: TCM-G804

Passage Ratio: 3×10⁵-5×10⁵ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection