Product Name：Luciferase Reporter MBT-2 Cell Line

HyCyte cell line：HyCyte MBT-2-Luc

Catalog No.：TCM-C802L

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：MBT-2

Species：Mouse

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium: RPMI-1640 supplemented with 10 % FBS,4.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G802L).

Passage：1:4-1:6

Doubling time：24-36 h

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).