The J774A.1 cell line is derived from BABL/cN mice and exhibits antibody-dependent phagocytosis. Its growth is inhibited by dextran sulfate, PPD, and LPS. J774A.1 cells can produce IL-1β and large amounts of lysozyme.

Cell Name: Mouse monocyte/macrophage cell line

Cell Synonyms: J774A.1; J-774A.1; J774.A1; J774 A1; J774A.1; J 774A.1; J774 A.1

Catalogue No.: TCM-C798
Species: Mouse
Tissue Origin: Blood
Disease Characteristic: Leukemia
Morphology: Macrophage-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G798

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection