The MH-S cell line is derived from the adherent cell-enriched population of mouse alveolar macrophages transformed by SV40. The cells retain many characteristics of alveolar macrophages, including the typical morphology of macrophages. They are adherent, phagocytic, esterase-positive, and peroxidase-negative. Treatment with lipopolysaccharide (LPS) can stimulate the production of IL-1. The cells are capable of inhibiting the in vitro plaque-forming cell (PFC) response in a cell dose-dependent manner.

Cell Name: Mouse alveolar macrophage cell line

Cell Synonyms: MH-S
Catalogue No.: TCM-C795
Species: Mouse
Tissue Origin: Lung
Disease Characteristics: —
Morphology: Macrophage-like
Growth Characteristics: Semi-adherent / semi-suspension
Culture Medium: RPMI-1640 + 10% FBS + 0.05 mM β-mercaptoethanol + 1% P/S

Matching Medium Catalog Number: TCM-G795

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection