In 1939, the Gl261 tumor was induced by intracranial injection of 3-methylcholanthrene, cultured in vivo in C57BL/6 mice, and maintained through serial transplantation in syngeneic mouse strains. In the mid-1990s, an in vitro cell culture system was established. The literature describes cells carrying TP53 and KRAS mutations.

Cell Name: Mouse glioma cell line

Cell Synonyms: GL261
Catalogue No.: TCM-C793
Species: Mouse
Tissue Origin: Glioma
Disease Characteristics: —
Morphology: Fibroblast-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS + 1% Glutamax + 1% P/S
Matching Medium Catalog Number: TCM-G793

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 28-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection