Product Name：Luciferase/GFP Dual-Reporter G422 Cell Line

HyCyte cell line：HyCyte G422-Luc-GFP

Catalog No.：TCM-C792LG

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：G422

Species：Mouse

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium： Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,5.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G792LG）

Passage：1:4-1:6

Doubling time：24-32 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).