Established in 1964; astrocytomas were induced by implanting methylcholanthrene into the brains of Km mice, and after passage to the 120th generation, the tumors transformed into glioblastomas. Subcutaneous, intramuscular, and intracerebral transplantation of the tumor cell suspension into syngeneic mice achieved a 100% success rate. Intramuscular and intracerebral transplantations resulted in relatively stable transplantable tumor strains, with survival times of 15.9 ± 4.8 days for the intracerebral type and 20.3 ± 6.6 days for the intramuscular type.

Cell Name: Mouse glioblastoma cell line

Cell Synonyms: G422
Catalogue No.: TCM-C792
Species: Mouse
Tissue Origin: Brain
Disease Characteristic: Glioblastoma Multiforme

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G792

Passage Ratio: 1:4-1:6, with medium renewal every 2-3 days
Doubling Time: 24-32 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection