Product Name：Luciferase Reporter S180 Cell Line

HyCyte cell line：HyCyte S180-Luc

Catalog No.：TCM-C789L

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：S180

Species：Mouse

Morphology：Lymphoblastoid

Growth properties：Attached or floating

Culture Medium: Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,15.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G789L）

Passage：1:2-1:4

Doubling time：24-32 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).