The H22 cell line was isolated from mouse ascites and established by the Dalian Medical College.

Cell Name: Mouse hepatocellular carcinoma cell line

Cell Synonyms: H22; Hepatoma-22; Hepatoma 22

Catalogue No.: TCM-C788

Species: Mouse

Tissue Origin: Liver

Disease Characteristic: Hepatocellular Carcinoma (Liver Cancer)

Morphology: Lymphoblast-like

Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G788

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection