Product Name:Luciferase Reporter C1498 Cell Line
HyCyte cell line:HyCyte C1498-Luc
Catalog No.:TCM-C785L
Construction method:Lentivirus
Selection Marker:Puromycin
Parental:C1498
Species:Mouse
Morphology:Lymphoblastoid
Growth properties:Suspension
Culture media: Dulbecco's Modified Eagle Medium ,High Glucose (DMEM) supplemented with 10 % FBS,1.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G785L)
Passage:1:3-1:6
Doubling time:48-72 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media:DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).