This cell line is derived from spermatocytes of a 6-week-old male BALB/c mouse, stably co-transfected with the SV40 large T antigen gene (pSV3-neo) and a temperature-sensitive p53 tumor suppressor gene (HTRp53cG9). The cells contain papovavirus.

Cell Name: Mouse spermatocyte cell line

Cell Synonyms: GC-2; GC-2spd(ts)

Catalogue No.: TCM-C782
Species: Mouse
Tissue Origin: Testis
Disease Characteristics: /

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS + 1% Glutamax + 1% P/S
Matching Medium Catalog Number: TCM-G782

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection