This cell line is derived from pSV3-neo transformed spermatogonia of a 10-day-old male BALB/c mouse. The cells contain SV40 viral DNA sequences.

Cell Name: Mouse spermatogonial cell line

Cell Synonyms: GC-1 spg; GC-1; GC1-SPG

Catalogue No.: TCM-C781
Species: Mouse
Tissue Origin: Testis
Disease Characteristics: /

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS + 1% Glutamax + 1% P/S
Matching Medium Catalog Number: TCM-G781

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection