The cAMP production in TM3 cells increases under the action of LH, but they do not respond to follicle-stimulating hormone (FSH). The duration of the response to LH is related to the batch of serum. In the presence of LH, the cells can metabolize cholesterol.

Cell Name: Mouse Leydig cell line

Cell Synonyms: TM3; TM-3

Catalogue No.: TCM-C776
Species: Mouse
Tissue Origin: Testicular interstitial
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM/F12 + 2.5% FBS + 5% Horse Serum + 1% P/S

Matching Medium Catalog Number: TCM-G776

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 16 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection