The staining of the cytoskeleton in SV40 MES 13 cells prominently highlights actin, with abundant parallel microfilaments present in the cytoplasm. It has been reported that SV40 MES 13 cells contract in the presence of 1 μM angiotensin II. SV40 MES 13 cells form colonies in soft agar; they are positive for the large T antigen of SV40.

Cell Name: Mouse mesangial cell line

Cell Synonyms: SV40 MES 13; SV40-MES13; MES-13; MES 13; MES13

Catalogue No.: TCM-C775

Species: Mouse

Tissue Origin: Glomerulus

Disease Characteristics: —

Morphology: Fibroblast-like

Growth Properties: Adherent growth

Culture Medium: DMEM-H : Ham's F-12 (mixed at a 3:1 ratio) + 5% FBS + 1% P/S

Matching Medium Catalog Number: TCM-G775

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 16-30 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection