Product Name：Luciferase Reporter Py8119 Cell Line

HyCyte cell line：HyCyte Py8119-Luc

Catalog No.：TCM-C764L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：Py8119

Species：Mouse

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Ham's F-12K supplemented with 5 % HS,8.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G764L).

Passage：1:3-1:6

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：Ham's F-12K+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).