Culture Medium:F12K+5%FBS+ 1% P/S Matching Medium Catalog Number: TCM-G764 Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days Doubling Time: 24-48 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection