The P388D1 cell line is derived from a lymphoma induced by methylcholanthrene. Upon induction with LPS and phorbol ester, P388D1 cells can produce IL-1 as well as lysozyme. These cells are capable of phagocytosing yeast polysaccharides and latex beads, and exhibit activity in the antibody-dependent, cell-mediated cytotoxicity (ADCC) system. The P388D1 cells are negative for surface immunoglobulin (sIg) and free of ectromelia virus.

Cell Name: Mouse lymphocytic neoplasm cell line

Cell Synonyms: P388D1; P-388D1; P388-D1; P388.D1; P3 88 D1

Catalogue No.: TCM-C757
Species: Mouse

Tissue Origin: Lymphoid
Disease Characteristic: Lymphoma
Morphology: Lymphoblast-like
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G757

Passage Ratio: 2-5×10⁵ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection