NIH/3T3 is a highly contact-inhibited, continuous cell line established from the embryonic cultures of NIH Swiss mice. To develop sublines more suitable for transformation analysis in terms of morphological characteristics, the original NIH/3T3 cell line underwent more than five rounds of subcloning. This cell line is highly sensitive to the formation of transformed foci by sarcoma viruses and the replication of leukemia viruses, making it very useful for studies on DNA transformation and transfection. It is negative for ectromelia virus (mousepox virus).

Cell Name: Mouse embryonic fibroblast cell line

Cell Synonyms: NIH-3T3; NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

Catalogue No.: TCM-C752
Species: Mouse
Tissue Origin: Embryo
Disease Characteristics: /
Morphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% CS (Newborn Calf Serum) + 1% P/S

Matching Medium Catalog Number: TCM-G752

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection