The NCTC clone 929 (mouse connective tissue), a clone of cell line L, was established in March 1948. Cell line L is one of the earlier continuously cultured cell lines, and clone 929 is one of the early clonal strains. The parental cell line L was derived from the normal subcutaneous loose connective tissue and adipose tissue of a 100-day-old male C3H/An mouse. Clone 929 was established by isolating single cells from passage 95 of cell line L using the capillary method. Testing confirmed that the cells were negative for ectromelia virus.

Cell Name: Mouse connective tissue fibroblast

Cell Synonyms: L-929; NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells;   L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC);   Clone 929; Earles's cells; Earle's L cells

Catalogue No.: TCM-C749

Species: Mouse
Tissue Source: Subcutaneous connective tissue
Disease Characteristics: /
Morphology: Fibroblast-like
Growth Characteristics: Adherent growth
Culture System: MEM + 10% Horse Serum + 1% P/S
Matching Medium No.: TCM-G749

Passage Ratio:: 1:3–1:6, change medium every 2–3 days

Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma