This cell line was established in 1992 by Kathryn A. Wikenheiser in transgenic mice, where the SV40 large T antigen, regulated by the promoter region of the human surfactant protein C gene, induced lung tumors in the mice.

Cell Name: Mouse lung epithelial cell line

Cell Synonyms: MLE-12; MLE12

Catalogue No.: TCM-C745

Species: Mouse
Tissue Origin: Lung
Disease Characteristics: /
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture System: DF-12 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCM-G745
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma