MFC cells were established by Qian Shusen and Gao Jin in 1985. They were cultured using small tissue blocks from FC tumor tissue derived from a transplanted forestomach squamous carcinoma in 615 mice and have been passaged for 132 generations. MFC cells achieve a 100% success rate when transplanted subcutaneously in 615 mice.

Cell Name: Mouse Gastric Cancer Cells
Cell Synonyms: MFC
Catalogue No.: TCM-C744
Species : Mouse
Tissue Origin: Stomach
Disease Characteristics: Gastric Cancer
Morphology: Epithelial-like
Growth Properties: Adherent Growth
Culture Medium: RPMI-1640 + 10% FBS (Fetal Bovine Serum) + 1% P/S
Passage Ratio: 1:2-1:4, with medium change every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommendation: Use HyCyte® One-Step Freezing Solution (ready-to-use, serum-free, no programmed cooling required), Product ID: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma.
Note: Cells should be passaged every 2-3 days.Split at 80-85% confluency, approximately 1:2-1:4 （based on flask bottom area）.