The L5178Y TK+/- clone (3.7.2C) cell line is a derivative of the L5178Y-S cell line.

Cell Name: Mouse lymphoma cell line

Cell Synonyms: L5178Y TK+/- CLONE (3.7.2C); L5178Y TK+/-3.7.2c; TK+/- (clone 3.7.2C)

Catalogue No.: TCM-C740

Species: Mouse

Tissue Origin: Lymphoma

Disease Characteristics: Lymphoma

Morphology: Lymphoblast-like

Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G740

Passage Ratio: 3×10⁵-1×10⁶ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection