The L Wnt-3A is a stable transfectant derived from L-M(TK-) cells by transfection with a Wnt-3A expression vector and subsequent selection in medium containing G418. The Wnt-3A gene encodes a secreted glycoprotein with altered signaling activity. Wnt genes regulate many pattern formation and growth events during embryonic development. L Wnt-3A cells secrete biologically active Wnt-3A protein, making them the best current source for producing Wnt-3A conditioned medium. Since this conditioned medium also contains other factors besides Wnt-3A protein, it is necessary to use the parental cell line as a control in experiments involving Wnt-3A conditioned medium.

Cell Name: Mouse fibroblast cell line secreting Wnt-3A

Cell Synonyms: L Wnt-3A; L-Wnt-3A; L-Wnt3A; LWnt3A; LWnt-3A

Catalogue No.: TCM-C738
Species: Mouse
Tissue Origin: Subcutaneous connective
Disease Characteristics: —
Morphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 400 µg/mL G418 + 1% P/S
Matching Medium Catalog Number: TCM-G738

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection