Antigen expression: CD31 positive [PubMed:1315100]
Products: Factor VIII [PubMed: 11315100]; Bone sialoprotein (BSP) [PubMed: 11315100]; Biglycan [PubMed: 11315100]; Decorin [PubMed: 1315100]; Villin 2 (Ezrin) [PubMed: 11325848].
The expression of bone sialoprotein, biglycan, decorin, and osteopontin indicates its characteristics as an osteogenic family cell. This cell line has been tested for mycoplasma contamination.

Cell Name: Mouse osteosarcoma cell line, wild type

Cell Synonyms: K7M2-WT; K7M2 wt; K7M2

Catalogue No.: TCM-C737
Species: Mouse
Tissue Origin: Bone
Disease Characteristics: Osteosarcoma
Morphology: Osteoblast
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCM-G737

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 31 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection