This cell line was derived from a BW7756 hepatoma induced in C57/L mice; it expresses AFP (alpha-fetoprotein), α1-antitrypsin, and amylase; and is negative for ectromelia virus. This cell can proliferate in serum-free medium with the following composition: 75% DMEM, 25% Waymouth’s MAB87/3 medium, supplemented with 3×10⁻⁸ M selenium.

Cell Name: Mouse hepatocellular carcinoma cell line

Cell Synonyms: Hepa1-6; HEPA 1-6; Hepa-1-6

Catalogue No.: TCM-C734

Species: Mouse
Tissue Origin: Liver
Disease Characteristic: Hepatocellular Carcinoma (Liver Cancer)
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: DMEM-H + 10% FBS + 1% Glutamax + 1% Sodium Pyruvate + 1% P/S
Recommended Medium No.: TCM-G734
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma