Product Name：Luciferase Reporter EMT-6 Cell Line

HyCyte cell line：HyCyte EMT-6-Luc

Catalog No.：TCM-C728L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：EMT-6

Species：Mouse

Morphology：Epithelial-like

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 15 % FBS,2.0ug/mL Puromycin,   2mM Glutamax,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G728L).

Passage：1:4-1:8

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).