The EMT-6 cell line was established from a transplantable mouse mammary carcinoma that arose following the implantation of hyperplastic mammary nodules in BALB/cCrGL mice. The resulting tumor line (designated KHJJ) was propagated in BALB/cKa mice and, after 25 animal passages, was adapted to tissue culture and named EMT. EMT-6 is a clonal isolate of EMT that was separated at Stanford University in 1971.

Cell Name: Mouse mammary carcinoma cell line

Cell Synonyms: EMT-6; Experimental Mammary Tumour-6

Catalogue No.: TCM-C728

Species: Mouse
Tissue Source: Mammary Gland
Disease Characteristics: Breast Cancer
Morphology: Epithelial-like
Growth Characteristics: Adherent
Culture System: RPMI-1640 + 15% FBS + 1% Glutamax + 1% P/S
Matching Medium Code: TCM-G728
Passage Ratio: 1:4–1:8; change medium every 2–3 days
Doubling Time: 24–48 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma