Product Name：Luciferase Reporter EL4 Cell Line

HyCyte cell line：HyCyte EL4-Luc

Catalog No.：TCM-C727L

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：EL4

Species：Mouse

Morphology：Lymphoblastoid

Growth properties：Suspension

Culture Medium:Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,6.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G727L）

Passage：3×10^5-5×10^5cells/mL

Doubling time： 24-48 h

Subculture： Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).