This cell is a cytotoxic T lymphocyte derived from C57BL/6. Its growth is dependent on IL-2.

Cell Name: Mouse cytotoxic T cell line

Cell Synonyms: CTLL-2; CTLL 2; CTLL2; CTLL(2)

Catalogue No.: TCM-C724
Species: Mouse
Tissue Origin: Lymphocyte
Disease Characteristics: /
Morphology: Lymphocyte-like
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS +   100 U/mL mouse Recombinant IL-2 + 1.0 μg/mL Con A + 1% P/S
Matching Medium Catalog Number: TCM-G724

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 72-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection