The CT26 cell line is an undifferentiated mouse colon cancer cell line induced by N-nitroso-N-methylurethane (NNMU). A clone derived from this cell line was named CT26.WT. The CT26.WT cells were stably transformed using a retroviral vector, LXSN, to generate a lethal subclone called CT26.CL25. This viral vector carries the lacZ gene, which encodes both a tumor-associated antigen (TAA) and beta-galactosidase. CT26.WT and CT26.CL25 cells exhibit similar growth rates and lethality in mice. However, CT26.CL25 cells can express the tumor-associated antigen and beta-galactosidase, making these two cell lines suitable for combined use in studies on immunotherapy and host immune responses.

Cell Name: Mouse colorectal carcinoma cell line

Cell Synonyms: CT26; CT-26; CT 26; CT-26 WT

Catalogue No.: TCM-C723
Species: Mouse
Tissue Origin: Colon
Disease Characteristics: Colon Cancer
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G723

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection